20-Hydroxyecdysone stimulates the accumulation of translatable yolk polypeptide gene transcript in adult male Drosophila melanogaster.
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چکیده
منابع مشابه
20-hydroxyecdysone stimulates tissue-specific yolk-protein gene transcription in both male and female Drosophila.
The yolk polypeptides of Drosophila are normally synthesized in the fat body and ovarian follicle cells of adult females. In response to 20-hydroxyecdysone males synthesize yolk polypeptides. The actual level of yolk polypeptides synthesized in males is not always a direct reflection of the YP-transcripts present. Initially YP-transcripts are efficiently translated into polypeptides whereas lat...
متن کاملDietary components modulate yolk protein gene transcription in Drosophila melanogaster.
The three yolk proteins of Drosophila melanogaster begin to be synthesized at eclosion. Transcription of the genes is regulated by the genes tra, tra-2 and dsx and also by the insect hormones, juvenile hormone and 20-hydroxyecdysone. We show that there is yet another level of control which is dependent upon feeding. Females that are starved from eclosion show a basal level of yolk protein gene ...
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In holometabolous insects, the steroid hormone 20-hydroxyecdysone (20E), in coordination with juvenile hormone, regulates the major developmental events that promote larval development and the transition from the larval to the pupal stage. Intimately entwined with the hormonal control of development is the control of larval growth and the acquisition of energy stores necessary for the developme...
متن کامل20-Hydroxyecdysone increases levels of transient gene expression in transfected Drosophila cells.
Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, in situ, this increase appears to be due to an increase in the number of cells...
متن کاملVitellogenin in Drosophila melanogaster: sequence of the yolk protein I gene and its flanking regions.
We have isolated recombinant DNA clones coding for female specific proteins from Drosophila melanogaster. By screening with 32P-(A)+RNA from male and female flies, respectively, we were able to isolate a set of 100 cDNA clones which showed a positive hybridization signal for RNA from female flies. These clones have been rescreened with RNA isolated from fat body of two day old male and female f...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.1.186